Why is polymerase chain reaction (PCR) crucial in biotechnology?

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Polymerase chain reaction (PCR) is a fundamental technique in biotechnology because it allows for the rapid amplification of specific DNA sequences. By increasing the quantity of DNA, researchers can create millions of copies of a particular segment, which is essential for various applications, including genetic analysis, cloning, medical diagnostics, and forensic science.

The ability to obtain sufficient amounts of DNA is critical, especially when starting with very small samples, as it enables scientists to perform various analyses and manipulations that would otherwise be impossible. This amplification process involves repeated cycles of denaturation, annealing, and extension, which result in exponential increases of the target DNA sequence.

In contrast, the other options relate to different processes that are not directly linked to the primary function of PCR. For instance, synthesizing new RNA molecules is associated with transcription, while reducing the quantity of DNA directly contradicts the purpose of PCR. Lastly, the creation of proteins from DNA sequences pertains to translation and involves processes like transcription and RNA processing, rather than the amplification of DNA itself.

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