What can be determined if the absorbance of an unknown sample is known and a standard curve is used?

Using a standard curve, the concentration of an unknown sample can be determined based on its absorbance. A standard curve is created by plotting the absorbance values of known concentrations of a substance, allowing for a visual representation of the relationship between absorbance and concentration according to Beer-Lambert Law.

When the absorbance of the unknown sample is measured, it can be compared to the standard curve to interpolate the corresponding concentration. This method is widely used in various fields, including biomedicine, to quantify the concentration of substances in a solution based on their light absorbance properties.

The other options, such as pH, volume, or molarity of the solvent, do not directly correlate with the absorbance measurement in this context. The standard curve specifically relates absorbance to concentration, making it a key tool for determining how much of a substance is present in a sample.